New Paper: High-Frequency Microfluidic Fractionation for Compound-Resolved Bioactivity-Based Metabolomics
- Daniel Petras
- Oct 27
- 1 min read
We are super excited that our work on microfluidic-based LC-MS/MS fractionation, in combination with bioluminescence bioreporters for compound-resolved bioactivity metabolomics, was just published:
Making use of high-speed, low-volume fractionation, we can achieve similar sampling rates for bioactivity measurements as for the mass spec DDA cycles, which enables correlation of LC-MS and LC-bioactivity datapoints to identify bioactive metabolites.
This was a large interdisciplinary effort together with the group of Heike Broetz-Oesterhelt within the CMFI Cluster in Tuebingen, and Mingxun Wang at UC Riverside. We started the project four years ago, when Albert Hernandez, a mechanical engineer from UC San Diego, joined us in Tuebingen, and built the microspotter hardware.
Combining the new tool with the bioluminescent bioreporter strains from Heike Broetz-Oesterhelt and her students Julian Schubert and Leonardo Boldt was a key element to achieve the high sensitivity of the bioactivity assays needed for microfractions.
Another key component was the analysis software, which Simon Knoblauch, back then a master’s student in Tuebingen, built and which, thanks to the help of Mingxun Wang, is now available in GNPS2. Most importantly, the paper would not have been possible without Christian Geibel, who led the project, optimized and validated the method, pushed the paper over the finish line, and contributed the soundtrack for our microspotter video:
Thanks a lot Christian and everybody else at the University of Tuebingen and UC Riverside who were involved in the project 🧡🧡🧡
Comments